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KMID : 1094720070120060720
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Biotechnology and Bioprocess Engineering
2007 Volume.12 No. 6 p.720 ~ p.729
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Investigation of the physiological properties and synthesis of PUFAs from Thraustochytrids and its electrophoretic karyotypes
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Anbu Periasamy
Kim Dong-Uk
Jeh Eun-Jin
Jeong Young-Su
Hur Byung-Ki
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Abstract
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Physiological properties of organism, such as the number of chromosomes, genome size, fatty acid profile and the activities of desaturases and elongases were investigated for four differentThraustochytrium species. The investigation revealed thatThraustochytrium aureum could synthesize a significant level of polyunsaturated fatty acids (PUFAs), particularly docosa-hexaenoic acid (DHA), when compared to the other threeThraustochytrium species tested. A higher level of saturated fatty acids was observed byT. striatum followed byThraustochytrium sp. ATCC 26185. The PUFA accumulation rate was higher in the n-3 than in the n-6 pathway. A comparison of the activities for these desaturases and elongases of the four different species were also studied. Further, the electrophoretic karyotypes of Thraustochytrids were separated by pulsed-field gel electrophoresis (PFGE). The separation condition of PFGE was developed to obtain the different chromosomes from the variousThraustochytrium species. The number of chromosomes inT. aureum, T. striatum, Thraustochytrium sp. ATCC 20891 andThraustochytrium sp. ATCC 26185 were 13, 17, 10. 8, and the whole genome size of those species were estimated to be 12.9, 11.7, 11.3 and 9.93 Mbp, respectively.
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KEYWORD
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chromosome, polyunsaturated fatty acids, fatty acid profiles, pulsed-field gel electrophoresis, Thraustochytriumspecies
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